tpaull Posted March 5, 2021 Share Posted March 5, 2021 I already posted this elsewhere but did not get a response, so posting again here. I am opening a ~8 MB greyscale tiff file but it opens in AP with very high contrast and low resolution, as if the greyscale detail has been lost. How do I fix this? tp030321.tif Quote Link to comment Share on other sites More sharing options...
Old Bruce Posted March 5, 2021 Share Posted March 5, 2021 I am thinking the Typhoon FLA 9500 (?) messed up the export of the file to TIFF. Quote Mac Pro (Late 2013) Mac OS 12.7.6 Affinity Designer 2.5.5 | Affinity Photo 2.5.5 | Affinity Publisher 2.5.5 | Beta versions as they appear. I have never mastered color management, period, so I cannot help with that. Link to comment Share on other sites More sharing options...
tpaull Posted March 5, 2021 Author Share Posted March 5, 2021 There are pixelation/resolution problems opening tiff files generated by other instruments though. Not specific to the typhoon. Quote Link to comment Share on other sites More sharing options...
Old Bruce Posted March 5, 2021 Share Posted March 5, 2021 2 minutes ago, tpaull said: There are pixelation/resolution problems opening tiff files generated by other instruments though. Not specific to the typhoon. Do you have the ability to set the export settings for all these instruments? Choose a colour space and high bit depth for all of them. Say.... 16 bit Greyscale, D50. Quote Mac Pro (Late 2013) Mac OS 12.7.6 Affinity Designer 2.5.5 | Affinity Photo 2.5.5 | Affinity Publisher 2.5.5 | Beta versions as they appear. I have never mastered color management, period, so I cannot help with that. Link to comment Share on other sites More sharing options...
tpaull Posted March 5, 2021 Author Share Posted March 5, 2021 I don't think so but I'll check. What is the significance of the pop up window that AP shows when the files are opening? (attached) If I change the profile, will this improve the resolution? assigned profile pop up.docx Quote Link to comment Share on other sites More sharing options...
kirkt Posted March 5, 2021 Share Posted March 5, 2021 The image does not have an embedded grayscale profile, and the overall interpretation of the grayscale values in the data can vary depending on the profile that is used for display. You might want to check the Typhoon FLA scanner software to see if it has the ability to export the TIFF with a particular profile embedded (tagged) in the exported file. Either that, or see if the scanner software user manual explains how the grayscale image data are formatted (for example, the gamma of the grayscale encoded data) so that you can ASSIGN that particular profile within AP. User Manual: https://www.dbi.udel.edu/wp-content/uploads/2017/07/Typhoon-FLA-9500-UserManual.pdf According to this literature: https://rega.kuleuven.be/bac/economou/files/pdf/manuals/typhoon-fla9500-biomolecular-imager.pdf the output files are stored in two different formats: Quote Data storage Data are stored either in linear 16-bit grayscale TIFF (.TIF file format) or in square root encoded 16-bit TIFF (.GEL file format). The .GEL format encoding provides higher dynamic resolution than .TIF at lower signal levels to exploit the low signal detection capability of the phosphorimaging technology. The dialog warning that AP displays is telling you that the image you are opening has no embedded profile and that AP is assigning one, by default (based on your color settings in the Preferences). This may not be the correct profile to interpret the image data, so the appearance of the image may not be what you expect in AP until you assign the correct profile. kirk Quote Link to comment Share on other sites More sharing options...
tpaull Posted March 5, 2021 Author Share Posted March 5, 2021 OK, thanks. It is true that opening the gel file instead of the tiff export gives a better match to the original. It also helps to change the color profile in AP first to 16-bit grey; this allows for similar color as the original also. I think this problem is reasonably solved now. kirkt 1 Quote Link to comment Share on other sites More sharing options...
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