Thank you for responding Lee.
I have renamed the folder, then opened Publisher and the Resource Manager says the files are missing. The screen shot I have attached shows the Resource Manager at this stage. You mention then to delete the resources. This I am not sure of as the resources are now in a renamed folder and obviously I don't want to delete these. The Resource Manager doesn't seem to have a delete function - or am I just not seeing it?
The file is actually multiple files as there are a lot of images and each image is saved in a folder of that animal - i.e. Tiger images are saved in a folder titled Tiger, and this folder is within a Parent folder- thus with all the animal images etc.
The files are on my system, not a removeable drive.
Sorry to be a pain, but please can you clarify the suggestion to delete the resources? In the meantime, I shall make a new folder for all of my resources in preparation to linking to them.